F-actin in control EpH4 cells. EpH4 cells stably expressing Lifeact-EGFP were transfected with control siRNA for 3 d and imaged by a spinning-disc laser confocal microscope (IX71; Olympus) equipped with CSU-X1 (Yokogawa Electric Corporation) and an UplanSApo 60×/1.35 oil lens (Olympus). Images were taken every 2 min for 36 min with Z-stacks of 0.25-µm thickness. Pictures at each time point were Z-projected. Frame rate of the video is 6 frames/s. Bar, 10 µm. Related to Fig. 3 C.
