sema3d morphants show impaired CCV development. Confocal time-lapse movie of CCV development in sema3d MO-injected Tg(kdrl:EGFP)s843 embryos from 28 to 32 hpf. CCV ECs migrate ventrally in a fanned-out manner. The EC sheet is disrupted. A confocal stack was taken every 15 min.
