Appearance of damaged lysosomes in scav-3 mutants expressing sfGFP::Gal3 and GBA-3::mCherry. Lysosomes in the hypodermis of a scav-3(qx193) adult expressing GBA-3::mCherry and sfGFP::Gal3 are followed. sfGFP::Gal3 clusters around the GBA-3::mCherry vesicle, and its enrichment is accompanied by a gradual loss of mCherry fluorescence. Images were analyzed by time-lapse confocal microscopy using an inverted fluorescence microscope with an UltraView spinning-disc confocal scanner unit. The frames were taken every 1 min for 1–3 h, and selected frames are displayed every 1 s. Selected images are shown in Fig. 6 B.
