Docking of CD63-positive MVEs at the PM (shallow TIRF angle) in SCC61 Rab27a-KD1 cells. 1 frame per 100 ms interval was taken for up to 30 s. Filled arrowheads point to CD63 puncta that are immobile for ≥5 s. Note decreased number of total and immobile puncta. Bar, 10 µm.