EEs co-migrate with moving peroxisomes. GFP-RabA/5a–labeled EEs (green) and mCherry-PTS1–labeled peroxisomes (magenta) in a WT hypha. White arrow indicates comigrating puncta. Both channels were acquired simultaneously and analyzed by time-lapse epifluorescence microscopy using a Deltavision OMX microscope. Frames were taken every 150 ms for 10.5 s. Video frame rate is 15 frames/s. This video was used to generate the kymograph in Fig. 3 E and stills in Fig. 3 F. Bar, 5 µm.
