PxdA co-migrates at the leading edge of moving peroxisomes. PxdA-GFP (green) and mCherry-PTS1–labeled peroxisome (magenta) dynamics in a WT hypha. Both channels were acquired simultaneously and analyzed by time-lapse epifluorescence microscopy using a Deltavision OMX microscope. Frames were taken every 250 ms for 13.75 s. Video frame rate is 15 frames/s. This video was used to generate the kymograph in Fig. 2 D (left) and stills in Fig. 2 E. Bar, 5 µm.
