EE movement in WT hyphae. EE (GFP-RabA/5a) movement and dynamics in a WT hypha. Images were analyzed by time-lapse epifluorescence microscopy using a Ti microscope (Nikon). Frames were taken every 150 ms for 10 s. Video frame rate is 15 frames/s. Similar videos were used for kymographs generated in Fig. 1 F.