Podosomal MT1-MMP persists beyond disruption of the podosome structure itself (from Fig. 5, A–F). TIRF time-lapse video of a primary human macrophage expressing MT1-MMP-pHluorin (green; exposure time at 488 nm, 600 ms) and Lifeact-RFP (red; exposure time at 568 nm, 50 ms). Acquisition rate: two time points/min; frame rate: 10 fps; sequence: 25 min and 6 s. Bar, 10 µm. Note the persistence of MT1-MMP signals even after CK-666–induced disruption of podosomes, indicated by absence of the F-actin reporter Lifeact-RFP.
