Asp-dependent focusing of intraspindle MTs. AspΔC-GFP (top) or Asp(FL)-GFP (bottom) and mCherry-tubulin were imaged every 4 s with spinning-disk confocal microscopy in Asp RNAi–treated cells. Arrowheads indicate the minus ends of the outermost MT bundle, whereas arrows indicate the pole. Time is displayed in minutes/seconds. Bar, 2 µm.
