Video of GFP:Utr-CH kinetics in an en passant synaptic bouton. Left bouton corresponding to Fig. 7 A. Greyscale frames of GFP:Utr-CH on left and corresponding overlaid frames of GFP:Utr-CH (green)/synaptophysin-mRFP (red) on right. Frame rate was 1 frame/s and playback rate is 10 frames/s. Total time of the movie is 600 s. Note transient patches of F-actin circumferentially surrounding the synaptic vesicle cluster (labeled by synaptophysin).
