Time-lapse monitoring of early-to-late endosome conversion in coelomocytes of N2, sorf-1(tm3855), sorf-2(tm5210), and sorf-1;sorf-2 animals. Early and late endosomes were labeled with 2xFYVE::GFP and mCherry::RAB-7, respectively. Images were taken every 30 s with a Z-series of 30 sections (0.5 µm/section) for 1 h using a DeltaVision microscopy system (DV Elite). Video was generated by projection of 20 sections. Image frames are shown at 8 fps. Images shown in Fig. 3 (A–D) were derived from this image sequence.
