High-resolution time-lapse imaging of Dlc1 and p190RhoGAP KD growth cones in N1E-115 cells. Lifeact-mCherry (F-actin) was acquired by time-lapse epifluorescence microcopy using a Nikon Eclipse Ti microscope at one frame every 5 s. Movie plays at 2 fps. Bar, 5 µm. Time scale, min:s.