Nuclei and somata segmentation evaluation. The ability of our method to segment nuclei and somata was evaluated using a set of three hand-annotated movies from the screen. A total of 790 cells were annotated and then compared with the corresponding automatic segmentation. The annotation is shown as a red area, and the automatic segmentation is depicted by the blue area. Quantifications of correct detections, false positives, and false negatives are shown in Fig. S2.
