Single ablation in S. pombe cells expressing Cnp1-GFP. (left) Live-cell microscopy of a S. pombe mitotic cell and the severing of the spindle. The cell expressing Cnp1 labeled with GFP (green) and α-tubulin labeled with mCherry (magenta) is shown. Images were acquired at 3.2-s intervals. The video is displayed at 10 fps. Bar, 3 µm. The video corresponds to Fig. 2 D. (right) Separate green channel showing S. pombe KTs (Cnp1-GFP).
