Single ablation in S. pombe cells expressing Ndc80-GFP. (left) Live-cell microscopy of a S. pombe mitotic cell and the severing of the spindle. The cell expressing Ndc80 labeled with GFP (green) and α-tubulin labeled with mCherry (magenta) is shown. Images were acquired at 2.8-s intervals. The video is displayed at 10 fps. Bar, 3 µm. The video corresponds to Fig. 1 E. (right) Separate green channel showing S. pombe KTs (Ndc80-GFP).
