Single ablation in PtK1 cells expressing Hec1-GFP. (top) Live-cell microscopy of a PtK1 mitotic cell and the severing of one of the two K-fibers. The cell expressing Hec1 labeled with GFP (green) was injected with α-tubulin-X-Rhodamine (magenta). Images were acquired at 2.5-s intervals. The video is displayed at 10 fps. Bar, 5 µm. The video corresponds to Fig. 1 B. (bottom) Separate green channel showing PtK1 KTs (Hec1-GFP).
