HSV-1 capsid movement in EB1- or CLIP-170–depleted NHDFs. NHDFs were treated with EB1 siRNA #1 (left) or CLIP-170 siRNA #1 (right) and then infected with HSV-1 K26GFP (green). 3 hpi, nuclei were stained with Hoechst (blue). Cells were analyzed using time-lapse microscopy on a DMI6000B-AFC widefield microscope with a 100× objective using an InVivo environmental chamber at 37°C. Images were captured at 1 fps for ∼400 s. The playback speed of the video is 15 fps (i.e., ∼15× real speed). In EB1- or CLIP-170–depleted cells, viral particles remain at the cell periphery and fail to initiate long-range transport to the nucleus.
