Movement of lysosomes in DMSO- or ciliobrevin D–treated NHDFs. NHDFs were treated with DMSO solvent control (left) or 200-µM ciliobrevin D (CbD; right) for 1 h and then stained with LysoTracker (green) as described in Materials and methods section Antibodies, WB, IF, and imaging. Cells were analyzed using time-lapse microscopy on a DMI6000B-AFC widefield microscope with a 100× objective using an environmental chamber at 37°C. Images were captured at 2 fps for 50 s. The playback speed of the video is 30 fps (i.e., ∼15× real speed).
