Movement of lysosomes in NHDFs depleted of CLIP-170 or DCTN1. NHDFs were treated with CLIP-170 siRNA #1 (left) or DCTN1 siRNA #2 (right) and then treated with LysoTracker (green) as described in Materials and methods section Antibodies, WB, IF, and imaging. Cells were analyzed using time-lapse microscopy on a DMI6000B-AFC widefield microscope with a 100× objective using an environmental chamber at 37°C. Images were captured at 2 fps for 30–50 s. The playback speed of the video is 30 fps (i.e., ∼15× real speed).
