Movement of mitochondria in NHDFs depleted of CLIP-170 or DCTN1. NHDFs were treated with CLIP-170 siRNA #1 (left) or DCTN1 siRNA #2 (right) and then treated with MitoTracker (green) as described in Materials and methods section Antibodies, WB, IF, and imaging. Cells were analyzed using time-lapse microscopy on a DMI6000B-AFC widefield microscope with a 100× objective using an environmental chamber at 37°C. Images were captured at 0.3 fps for 3 min. The playback speed of the video is 15 fps (i.e., ∼50× real speed).
