Video 7.

Normal mitotic timing in a cmt-1(ok2879) mutant two-cell embryo with monopolar spindles. A cmt-1(ok2879);zyg-1RNAi mutant embryo expressing GFP::PH and mCh::H2B for visualization of the plasma membrane and chromatin, respectively (strain BHL608). The AB cell is on the top left. Note the absence of a mitotic delay as compared with Video 6. Images were generated by time-lapse microscopy on a DeltaVision Personal DV deconvolution microscope and processed by constrained, iterative deconvolution using the softWoRx software package. Each frame is 20 s.


TRIP13PCH-2 promotes Mad2 localization to unattached kinetochores in the spindle checkpoint response

Christian R. Nelson, Tom Hwang, Pin-Hsi Chen, and Needhi Bhalla

DOI: 10.1083/jcb.201505114
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