FA dynamics. Left, inverted confocal images of paxillin-GFP in migrating C2C12 Pcad Cdc42 shRNA (top) and C2C12 Pcad β-PIX shRNA (bottom) cells located at the first multicellular row; 1 frame/5 s for 15 min. Arrows indicate the migration direction. Lines in the first frame indicate nontransfected surrounding cells. Right, ratio images generated from the time lapse shown on the left to illustrate FA dynamics, with magenta showing the extension and yellow the loss of FAs. Green represents the FA area maintained during the analyzed period.
