Fusion of macropinosomes and tubular endolysosomes. Time-lapse video of a macrophage stimulated with M-CSF and LY after preloading endolysosomes with TRDx. (top) Phase contrast. (second Panel) LY fluorescence (inverted contrast). (third panel) TRDx fluorescence (inverted contrast). (bottom) LY/TRDx ratio image. LY-labeled macropinosomes are bright and TRDx-labeled endolysosomes are black. Macropinosomes were wrapped by tubular endolysosomes before disappearing into the endolysosomal compartment. A large macropinosome was wrapped by tubular endolysosomes, exchanged dyes, and then was resorbed into the tubular endolysosomal network. Frame intervals = 20 s (data of Fig. 7 B).
