BubR1 localizes on laser-induced DNA breaks. WT neuroblast expressing GFP::BubR1 (left, cyan on right) and H2A.Z::RFP (red). Image acquisition starts 20s before laser ablation, and the acquisition is paused while inducing chromosome breaks with a 355-nm pulsed laser and starts again. The arrows indicate the appearance of GFP::Bub3 signal on the breaks. Images were analyzed by a time-lapse confocal spinning disk microscope equipped with an Evolve emCCD camera. Frames were taken every 20 s. Time is given in minutes/seconds. The video corresponds to Fig. 2 E.
