The BubR1 truncated construct 330–762 mutated on E481 does not localize on DNA breaks during mitosis. WT neuroblast expressing GFP::BubR1 330–762 E481K (left, cyan on right) and H2A.Z::RFP (red) after I-CreI induction. Images were analyzed by a time-lapse confocal spinning disk microscope equipped with an Evolve emCCD camera. Frames were taken every 20 s. Time is given in minutes/seconds. The video corresponds to Fig. 1 C (right).
