The degradation of the synthetic APC/C substrate CycBNt::Bub3 is not delayed on DNA breaks in bubR1-KEN mutant. bubR1-KEN mutant neuroblast expressing GFP::CycBNt::Bub3 (left, cyan on right) and H2A.Z::RFP (red) after I-CreI expression. Images were analyzed by a time-lapse confocal spinning disk microscope equipped with an Evolve emCCD camera. Frames were taken every 20 s. Time is given in minutes/seconds. The video corresponds to Fig. 6 B (bottom).
