GFP-RhoB was expressed in HUVECs for 24 h. TNF-pretreated cells were stimulated with thrombin for at least 60 min and then analyzed by time-lapse confocal microscopy at 20-s interval during a 20-min period. Video is displayed at six frames per second. Yellow arrowhead points to vesicles moving toward the cell periphery. Red arrows point to vesicles moving away from cell periphery. Frames in Fig. 6 A.
