Successful GFA1 mRNP export event in mex67-5 cells. Video shows an example of a successful export event based on tracking of a GFA1 mRNP with 24xPP7 loops in the 3′ UTR bound by PP7-CP-3xYFP (green) from the nucleus to the cytoplasm. NPCs are marked by Ndc1-tdTomato (red). Images were acquired using a custom dual channel setup (see Materials and methods section Live cell imaging of mRNP export and image processing) at a frame rate of 67 Hz, equaling a time resolution of 15 ms. Video is provided at a 5× reduced rate (13 Hz). Image processing for visual analysis was performed using Fiji.
