Extended mRNP interactions with the NE in mex67-5. Video shows two GFA1 mRNPs with 24xPP7 loops in the 3′ UTR bound by PP7-CP-3xYFP (green) engaged with the NE that persists for the entire length of the 7.5-s video in a mex67-5 cell. NPCs are marked by Ndc1-tdTomato (red). Images were acquired using a custom dual channel setup (see Materials and methods section Live cell imaging of mRNP export and image processing) at a frame rate of 67 Hz, equaling a time resolution of 15 ms. Video is provided at a 5× reduced rate (13 Hz). Image processing for visual analysis was performed using Fiji. Data for Fig. 3 B were taken from this event.
