GFA1 mRNP observed to split and merge. Video shows a cytoplasmic GFA1 mRNP with 24xPP7 loops in the 3′ UTR bound by PP7-CP-3xYFP (green) in REF cells. The particle splits and merges, suggesting that multiple GFA1 mRNAs can assembly together. NPCs are marked by Ndc1-tdTomato (red). Images were acquired using a custom dual channel setup (see Materials and methods section Live cell imaging of mRNP export and image processing) at a frame rate of 67 Hz, equaling a time resolution of 15 ms. Video is provided at a 5× reduced rate (13 Hz). Image processing for visual analysis was performed using Fiji. Data for Fig. S1 D were taken from this event.
