Inhibition of mLgl::GFP PM targeting by rapamycin-induced PM recruitment of PJ::RFP in HEK293 cells. Subcellular relocalization of mLgl::GFP and FKBP12-PJ::RFP in HEK293 cells in response to rapamycin treatment. HEK293 cells were triple transfected with mLgl::GFP, FKBP12-PJ::RFP, and PM-FRB-CFP. 24 h after transfection, 5 µM rapamycin was added to the cells. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (A1; Nikon). Frames were taken every 20 s for 440 s.
