Time-lapse recording of Lgl::GFP and His2Av::RFP in follicular cells under hypoxia and reoxygenation. Dissected ovaries from a 3-d-old lgl::GFP his2Av::RFP female were imaged live in a micro chamber. Hypoxic (0.5% O2) gas was introduced into the chamber at 2.2 min of recording, and the steady gas flow persisted until 39.3 min. Hypoxic gas was then replaced by normal airflow for reoxygenation. The sudden shifts of focus of video frame at 2.2 and 39.3 min are caused by the chamber pressure fluctuations as a result of gas source switching. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope (LSM 510; Carl Zeiss). Frames were taken every 19 s for 48.8 min under hypoxia.
