Video 9.

cAMP impairs FPR-induced mitochondrial activation. PMNs were loaded with Rhod2 and incubated for 20 min with or without 1 µM cAMP-AM, and fMLP-induced mitochondrial Ca²⁺ uptake was recorded by fluorescence microscopy (DMI6000 B; Leica; objective: 100× oil, NA 1.30; DFC365 FX camera; Leica). Frames were taken every 1 s for 30 s.

mTOR and differential activation of mitochondria orchestrate neutrophil chemotaxis

Yi Bao, Carola Ledderose, Amelie F. Graf, Bianca Brix, Theresa Birsak, Albert Lee, Jingping Zhang, and Wolfgang G. Junger

DOI: 10.1083/jcb.201503066