Inhibition of mitochondria by CCCP reduces PMN chemotaxis in response to IL-8 or LTB4. PMNs were incubated for 10 min with or without 1 µM CCCP, and bright field images of chemotaxis toward a micropipette loaded with 10 µg/ml IL-8 or 1 µM LTB4 were recorded over time as described in the legend for Video 1 using video microscopy (DMI6000 B; Leica; objective: 20×, air, NA 0.40; DFC365 FX camera; Leica). Frames were taken every 20 s for 7 min.
