Movie of an acentriolar Plk4AID/AID;p53 shRNA cell expressing EGFP-Centrin. IAA was washed out at 0 h to allow Plk4 recovery and de novo centriole formation. Images were analyzed by time-lapse confocal microscopy using a laser-scanning confocal microscope. Shown in Fig. 8 A (Cell 2). One frame captured every 10 min; displayed at 10 frames/s.
