S100b-GFP+ve cells respond to ephrin-A3 with repulsive motion. Cells were isolated from hindlimb muscle of S100b-GFP mice (green), plated over stripes of immobilized ephrin-A3-Fc (red), and imaged every 10 min. GFP+ve cells, which would include but not be limited to terminal Schwann cells, show repulsive motion after contact with ephrin-S3 stripes. Images were acquired from a DMI-4000 inverted microscope (Leica) fitted with a stage top incubator and a Q-Imaging camera (Retiga) using MetaMorph software (Molecular Devices) to drive the automatic acquisition.
