Z stack taken through an NMJ in TA muscle of S100b-GFP mouse. Terminal Schwann cells are GFP+ (green) and stain positively with anti-EphA8 (red). The postsynaptic membrane is visualized with Alexa Fluor 633–conjugated α-bungarotoxin (cyan). Images were collected on an upright microscope (BX-61; Olympus) using ImageJ software (National Institutes of Health).
