Wild-type GFP-Sec4 vesicle tracking example from Fig. 1 (a and b). Bleach event of the entire bud occurs at 5.1 s. Newly formed vesicles can then be observed entering the bud, docking with the plasma membrane, and disappearing in a frame-to-frame event. Images were analyzed by time-lapse spinning disk confocal microscopy (DMI600B, Leica; CSU-X, Yokogawa Electric Corporation) using Slidebook 5.0 software (Intelligent Imaging Innovations). Each frame is ∼1.28 s.
