3T3 migration on suspended fiber, formation of fin-like protrusion versus TPH1 on suspended fiber, and no fin-like protrusion. 3T3 fibroblast was plated on fibronectin-coated fiber. Cell was imaged in phase contrast with a 20× objective (IX81; Olympus), one image every 30 s for 108 min. Cell displayed fin-like protrusion. Bar, 20 µm (top and middle). TPH1 cells were plated on fibronectin-coated fiber. Cells were imaged in phase contrast with a 20× objective (IX81; Olympus), one image every 30 s for 108 min. This cell type did not display fin-like protrusion. Bar, 20 µm (bottom).
