Video 1.

wt fibroblasts expressing the ratiometric Twitch-1 calcium sensor, imaged in FRET mode, showing the “flickers” in calcium. Twitch-1–positive cells were imaged on the DeltaVision platform as described in In vitro calcium imaging. Images were collected in 30-s intervals during a 20-min period. Bar, 30 µm.

Transmembrane proteoglycans control stretch-activated channels to set cytosolic calcium levels

Sandeep Gopal, Pernille Søgaard, Hinke A.B. Multhaupt, Csilla Pataki, Elena Okina, Xiaojie Xian, Mikael E. Pedersen, Troy Stevens, Oliver Griesbeck, Pyong Woo Park, Roger Pocock, and John R. Couchman

DOI: 10.1083/jcb.201501060