Mena forms a ring around the Tks5 core. MDA-MB-231 cells were transiently cotransfected with mCherry-Tks5 and GFP-Mena, cultured on unconjugated matrix for 24 h, and subjected to time-lapse video microscopy. Images were acquired every 15 s for 5 min and are displayed at 3/10 of a second per frame. GFP-Mena is depicted on the left and mCherry-Tks5 on the right. Bars, 10 µm.