Actin branching reaction, +cortactin+PI(3,4)P2-liposomes condition. TIRF microscopy movie showing polymerization reaction containing 0.75 µM 33% Oregon Green actin, 10 nM Arp2/3 complex, 50 nM GST-VCA, 50 nM cortactin, and 200 nM PI(3,4)P2-liposomes. Single-wavelength (488 nm) images were acquired using a Nikon TiE inverted microscope every 10 s with an exposure time of 50 ms and played back at 15 fps. Bar, 5 µm. This video corresponds to Fig. 6 A.
