Asymmetric segregation of BiFC complexes with HcRed-PTS1 in vps1 cells. Diploid vps1 cells expressing both Pex13-VN and Pex14-VC (green) and HcRed-PTS1 (red). Images were acquired using time-lapse epifluorescence microscopy on a widefield microscope (Axio Observer). Stacks were taken every 20 min for 14 h. Video runs at one frame per second.
