Segregation of de novo–formed peroxisomes in vps1/dnm1 cells. Haploid vps1/dnm1 cells with galactose-controllable Pex19 constitutively expressing HcRed-PTS1 (red) were grown in media containing galactose. Image acquisition started 4.5 h after first exposure to galactose. Images were acquired using time-lapse epifluorescence microscopy on a widefield microscope (Axio Observer). Stacks were taken every 10 min for 15 h. Video runs at one frame per second.
