Video 8.

Stoichiometry of individual IFNAR complexes. Single-step bleaching events of two IFN-induced IFNAR1/IFNAR2 dimers (magenta) imaged by TIRFM (Olympus IX71; frame rate, 30 Hz; playback, real time). For the upper dimer, DY647 (IFNAR2, blue) bleaches first, for the lower dimer, TMR (IFNAR1, red) bleaches first.

Receptor dimerization dynamics as a regulatory valve for plasticity of type I interferon signaling

Stephan Wilmes, Oliver Beutel, Zhi Li, Véronique Francois-Newton, Christian P. Richter, Dennis Janning, Cindy Kroll, Patrizia Hanhart, Katharina Hötte, Changjiang You, Gilles Uzé, Sandra Pellegrini, and Jacob Piehler

DOI: 10.1083/jcb.201412049