Junctional actin dynamics in MCF10A cells. MCF10A cells coexpressing control shRNA and LifeAct-GFP were induced with doxycycline and seeded into Matrigel. After 24 h, cell pairs were monitored by time-lapse confocal microscopy (LSM 700; Carl Zeiss) with a 60×, NA 1.4 objective lens for 8 min. Z stacks were taken every 36 s. The LifeAct-GFP signal of one individual frame is shown.
