VAMP8-carrying and perforin-containing vesicles have different spatial and temporal characteristics. TIRF microscopy time-lapse imaging (98 s) of a human CTLs transfected with VAMP8-TFP (green) and perforin-mCherry (red) upon contact with glass coverslip coated with anti-CD3 and anti-CD28 antibodies. Images were analyzed using an inverted Olympus Optical IX 70 microscope with an acquisition frequency of 10 Hz (100 ms per frame) and a display rate of four frames per second. Reference figure: Fig. 5. Arrowheads are VAMP8-TFP fusion events. Bar, 2.5 µm.
