Live-cell imaging of Coronin 1B siRNA-transfected cells. U2OS cells were transfected with Coronin 1B siRNA for 48 h and then imaged by time-lapse microscopy using an inverted microscope (TE-200; Nikon). Frames were taken every 7 min for 24 h. Cell morphology showing an increase in bi- and multipolarity is similar to WISp39 KD cells.
