Localization and dynamics of mCherry-CD63 and GFP-PI4KIIα in Rab27a-silenced Jurkat cells. Time-lapse confocal microscopy of live Rab27a-silenced Jurkat cells expressing both mCherry-CD63 (red) and GFP-PI4KIIα (green). Images were acquired using a laser-scanning confocal microscope (spectral FV 1000; Olympus). Frames were taken every 2.65 s for 3.7 min. The video plays at 3 fps. Bar, 2 µm. Selected frames from this video are shown in Fig. S5 B.
