GFP-Sac2 dynamics at the late-stage clathrin-coated pits. COS7 cells were transfected with GFP-Sac2 and CLC-mRFP. Live imaging by spinning-disk confocal microscopy (UltraView VoX system; PerkinElmer) demonstrates endocytic clathrin-coated pit dynamics showing recruitment of GFP-Sac2 (green) at clathrin-coated pits just when the clathrin signal (CLC-mRFP, red) is about to disappear. Bar, 2 µm.
